Human 14-3-3 beta qPCR Primer Pair
Cat: HP100083
Human 14-3-3 beta qPCR Primer Pair: 一般情報
遺伝子情報
製品情報
オリゴヌクレオチドの種類:
qPCR Primers
成分:
1 vial of lyophilized qPCR primer mix (1 nmol each primer, sufficient for 200 numbers of 25 μl reactions).
QPCRプライマーの説明:
Verified forward and reverse primers for analyzing the quantitative expression of gene.
アプリケーション & 品質管理
アプリケーション:
SYBR® Green-based quantitative real-time PCR (qPCR).
品質管理:
The primer mix has been verified to generate satisfactory qPCR data on Roche Applied-science LightCycler® 480 Ⅱ.
保存 & 配送
配送方法:
Lyophilized qPCR primer mix is shipped at ambiente temperatura
保存条件:
The lyophilized product is stable for one year from date of receipt when stored at -20℃.
The suspended product is stable for six months from date of receipt when stored at -20℃.
***Sino biological qEASY qPCR primer pairs are used for SYBR Green-based real-time RT-PCR, The primers are designed by using SBI's proprietary primer design algorithm. Our primer collection covers the entire human genomes. It can be widely applied in the quantitative analysis of gene expression.***
特徴とメリット
独特なプライマー設計
ゲノムDNAの増幅を効果的に回避するために、このプライマーペアには少なくとも１つのプライマーまたは産物がイントロンを横切るように、特定の遺伝子の異なる変異体の保存領域でプライマーを設計します。
厳格なスクリーニング検証プロセス
プラスミド標準品でqPCRプライマーの感度、増幅効率、および特異性をスクリーニングし、陽性組織または細胞で検証し確認します。
均一なPCR条件、操作が簡単で、時間とコストを節約します
~100%という増幅効率で、RNA定量の正確さを保証
Human 14-3-3 beta qPCR Primer Pair: 検証済の画像
YWHAB dissolution curves
YWHAB amplification curves
Human 14-3-3 beta qPCR Primer Pair: 別名
GW128 qPCR Primer Pairs, Human; HEL-S-1 qPCR Primer Pairs, Human; HS1 qPCR Primer Pairs, Human; KCIP-1 qPCR Primer Pairs, Human; YWHAA qPCR Primer Pairs, Human
14-3-3 beta 背景情報
14-3-3 beta / YWHAB is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 beta / YWHAB is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. 14-3-3 beta / YWHAB interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. 14-3-3 beta / YWHAB has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 beta / YWHAB binding negatively regulates RSK1 activity to maintain signal specificity and that association/dissociation of the 14-3-3beta-RSK1 complex is likely to be important for mitogen-mediated RSK1 activation.
完全な名称
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
Related Pathways
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Death Receptor Signaling -
TNF Signaling -
Actin Dynamics Signaling Pathway -
AKT Signaling Pathway -
B Cell Receptor Signaling Pathway -
MAPK-Erk Pathway
参考文献
- Tommerup N, et al. (1996) Assignment of the human genes encoding 14,3-3 Eta (YWHAH) to 22q12, 14-3-3 zeta (YWHAZ) to 2p25.1-p25.2, and 14-3-3 beta (YWHAB) to 20q13.1 by in situ hybridization. Genomics. 33(1): 149-50.
- Jin YH, et al. (2008) Sirt2 interacts with 14-3-3 beta/gamma and down-regulates the activity of p53. Biochem Biophys Res Commun. 368(3): 690-5.
- Sekimoto T, et al. (2004) 14-3-3 suppresses the nuclear localization of threonine 157-phosphorylated p27(Kip1). EMBO J. 23(9): 1934-42.
14-3-3 beta qPCRプライマーペア, Human, 関連製品
14-3-3 beta qPCRプライマーペア, Mouse, 関連製品
14-3-3 beta qPCRプライマーペア, Rat, 関連製品
14-3-3 beta qPCRプライマーペア, Rhesus, 関連製品
14-3-3 beta qPCRプライマーペア, Rabbit, 関連製品
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