Anti-PD-L1 Antibody

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Anti-PD-L1 Antibody (Rabbit Polyclonal 抗体) の製品情報

製品名
Anti-PD-L1 Antibody
検証済のアプリケーション
WB
交差反応
Reacts with: Mouse
特異性
Mouse PD-L1
免疫原
Recombinant Mouse PD-L1/B7-H1/CD274 Protein (Catalog#50010-M08H)
調製
Produced in rabbits immunized with purified, recombinant Mouse PD-L1/B7-H1/CD274 (rM PD-L1/B7-H1/CD274; Catalog#50010-M08H; NP_068693.1; Met1-Thr238). PD-L1/B7-H1/CD274 specific IgG was purified by Mouse PD-L1/B7-H1/CD274 affinity chromatography.
ソース
Polyclonal Rabbit IgG
精製
Protein A & Antigen Affinity
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-PD-L1 Antibody (Rabbit Polyclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-PD-L1 Antibody (Rabbit Polyclonal 抗体) の画像

Anti-CD274 rabbit monoclonal antibody at 1:500 dilution

Lane A: Mouse heart tissue lysate

Lysates/proteins at 30μg per lane.

Secondary

Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size:33 kDa

Observed band size:50 kDa

Anti-PD-L1 Antibody: 別名

Anti-A530045L16Rik Antibody; Anti-B7h1 Antibody; Anti-Pdcd1l1 Antibody; Anti-Pdcd1lg1 Antibody; Anti-Pdl1 Antibody

PD-L1 背景情報

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.
完全な名称
CD274 molecule
参考文献
  • Iwai Y, et al. (2002) Involvement of PD-L1 on tumor cells in the escape from host immune system and tumor immunotherapy by PD-L1 blockade. Proc Natl Acad Sci U S A. 99(19): 12293-7.
  • Ghebeh H, et al. (2006) The B7-H1 (PD-L1) T lymphocyte-inhibitory molecule is expressed in breast cancer patients with infiltrating ductal carcinoma: correlation with important high-risk prognostic factors. Neoplasia. 8(3): 190-8.
  • Salih HR, et al. (2006) The role of leukemia-derived B7-H1 (PD-L1) in tumor-T-cell interactions in humans. Exp Hematol. 34(7): 888-94.
  • Wilcox RA, et al. (2009) B7-H1 (PD-L1, CD274) suppresses host immunity in T-cell lymphoproliferative disorders. Blood. 114(10): 2149-58.
  • Ruggiero A, et al. (2009) Crystal structure of PD-L1, a ribosome inactivating protein from Phytolacca dioica L. leaves with the property to induce DNA cleavage. Biopolymers. 91(12): 1135-42.

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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