|Comments for pCMV3-SP-N-His:|
|• CMV promoter: bases 250-837
• Enhancer: bases 838-1385
• SV40 early promoter: bases 2330-2699
• Hygromycin ORF: bases 2717-3742
• pUC origin: bases 4385-5058
• Kanamycin ORF: bases 5132-5947
|Vector Type||Mammalian Expression Vector|
|Expression Method||Constitutive, Stable / Transient|
|Selection In Mammalian Cells||Hygromycin|
|Sequencing Primer||Forward: T7(TAATACGACTCACTATAGGG)； Reverse: BGH(TAGAAGGCACAGTCGAGG)|
pCMV3-SP-N-His is recommended for constructing the N-His tag secretary and membrane proteins expression vector which containing a naïve signal peptide. An universal signal peptide is used to instead the naïve signal peptide.
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein.
Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.