Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set

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Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set 製品情報

製品名
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set
検出原理
Solid Phase Sandwich ELISA
特異性
Quantitative determination of ZIKV ZIKV NS-1 protein
線形範囲
62.5-4000 pg/mL
ELISA 抗体ペアセット(すぐに使用できません)の成分
1. Capture Antibody 1 mg/mL of mouse anti-Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 μg/mL in PBS before coating. (Catalog: # 40544-MM03)
2. Detection Antibody 0.2 mg/mL of mouse anti-Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 40544-MM10)
3. Standard Each vial contains 248 ng of recombinant Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended.
製品概要
This Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set is a solid phase sandwich ELISA for quantitative determination of ZIKV ZIKV NS-1 protein . It contains ZIKV ZIKV NS-1 protein capture antibody, ZIKV ZIKV NS-1 protein detector antibody (HRP) and a highly purified HEK293-expressed recombinant ZIKV ZIKV NS-1 protein protein. This Pair Set is at affordable price for researchers.
配送方法
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
保存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ELISA Pair Set の画像

ZIKV NS-1 protein 背景情報

Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replicaiton. The possible effects of NS1 on hosts include: localization to host cell surface and secreted extracellularly, modelates signalling of innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies.
参考文献
  • Wong SS-Y, et al., Zika virus infectiondthe next wave after dengue?, Journal of the Formosan Medical Association (2016)
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