CD55/DAF Matched ELISA Antibody Pair Set,Mouse

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CD55/DAF Matched ELISA Antibody Pair Set,Mouse 製品情報

製品名
CD55/DAF Matched ELISA Antibody Pair Set,Mouse
検出原理
Solid Phase Sandwich ELISA
特異性
Quantitative determination of Mouse CD55/DAF
線形範囲
5.47-350 pg/mL
ELISA 抗体ペアセット(すぐに使用できません)の成分
1. Capture Antibody 1 mg/mL of rabbit anti-Mouse CD55/DAF monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating. (Catalog: # 50468-R084)
2. Detection Antibody 0.2 mg/mL of rabbit anti-Mouse CD55/DAF polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4℃). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 19 ng of recombinant Mouse CD55/DAF. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 350 pg/mL is recommended.
製品概要
This CD55/DAF Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD55/DAF . It contains Mouse CD55/DAF capture antibody, Mouse CD55/DAF detector antibody (HRP) and a highly purified HEK293-expressed recombinant Mouse CD55/DAF protein. This Pair Set is at affordable price for researchers.
配送方法
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
保存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.

CD55/DAF Matched ELISA Antibody Pair Set,Mouse の画像

Citation List

CD55/DAF Matched ELISA Antibody Pair Set,Mouse: 別名

Daf Matched ELISA Antibody Pair Set, Mouse; Daf-GPI Matched ELISA Antibody Pair Set, Mouse; Daf1 Matched ELISA Antibody Pair Set, Mouse; GPI-DAF Matched ELISA Antibody Pair Set, Mouse

CD55/DAF 背景情報

CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 3 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 7-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.
完全な名称
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Related Pathways
  • Complement Activation Pathways
    Complement Activation Pathways
参考文献
  • Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.
  • Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.
  • Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.
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