SIM SF Expression Medium (For SF9 and SF21, Serum free)

SIM SF 培养基可用于培养SF9细胞和SF21细胞的无蛋白无血清培养基。SIM SF培养基仅供实验研究,不可用于人体及动物。

培养基特点
SIM SF 培养基为澄清淡黄色透明液体,如果一旦发现培养基变得浑浊或出现沉淀,请勿使用。
保存条件
2~8℃避光保存,一旦过期,请勿使用。
稳定性及保质期
室温下一个星期内稳定。
自收到之日起, 2~8℃避光保存,六个月内稳定。
细胞冻存与复苏
细胞冻存:
  1. 选择处于对数生长期的细胞,保证活率大于90%。
  2. 制备细胞冻存液:45%新鲜培养基,45%条件培养基,10%DMSO。
  3. 200g离心5分钟,弃去上清。
  4. 用细胞冻存液重悬细胞,细胞冻存密度为1.2~2×107/ml/ml。
  5. 迅速转移重悬的细胞至无菌的冻存管中,每管1~2ml。
  6. 将冻存管放入程序降温盒中,-70℃冰箱放置过夜。
  7. 若冻存细胞需要长期保存,将其转移至液氮罐中。
细胞复苏:
  1. 将冻存管迅速转移至27℃水浴中,进行融化。
  2. 将融化的细胞转至无菌的含5-10ml新鲜培养基的无菌离心管中。
  3. 200g低速离心5分钟,缓慢倒掉上部培养基,不要将沉降的细胞悬起。
  4. 用10-20ml新鲜培养基重悬细胞,确保重悬后的细胞密度大于1×106/ml左右。
  5. 将重悬的细胞放入无菌培养瓶中,旋盖拧松,放在摇床中悬浮培养。
  6. 当细胞密度生长达到2~4×106/ml时,可以进行传代,传代时细胞接种密度为0.8-1×106/ml。
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