MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (His Tag)

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MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (His Tag): 製品情報

純度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Testing in progress
タンパク質の構築
A DNA sequence encoding the Nucleoprotein (Human betacoronavirus 2c EMC/2012) (AFS88943.1) (Met 1-Asp413) was fused with a polyhistidine tag at the C-terminus.
GenBank No.
発現ホスト
Baculovirus-Insect Cells
MERS-CoV
予測N末端
Met 1
分子量
The recombinant Nucleoprotein (Human betacoronavirus 2c EMC/2012) comprises 423 amino acids and has a predicted molecular mass of 46.4 kDa. It migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
バッファー
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% gly, pH 8.0.
1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (His Tag): 画像

MERS-CoV (NCoV / Novel coronavirus) Nucleoprotein / NP protein (His Tag): 別名

NP Protein, MERS-CoV

CoV Nucleoprotein 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronaviruses primarily cause respiratory and enteric diseases in mammals and birds. Coronaviruses can cause a range of symptoms varying from mild symptoms such as the common cold to more serious respiratory illnesses. They primarily cause respiratory and enteric diseases in mammals and birds. Coronavirus symptoms include rhinorrhea, sneezing, cough, nasal obstruction, bronchitis and so on. There are three main groups of coronaviruses: alpha, beta, and gamma. Proteins that contribute to the overall structure of all coronaviruses are the spike (S), envelope (E), membrane (M) and nucleoprotein (N). Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
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