NGF Protein, Human, Recombinant

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NGF Protein, Human, Recombinant: 製品情報

純度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.
タンパク質の構築
A DNA sequence encoding the mature form of human β-NGF (NP_002497.2) (Ser 122-Arg 239) was expressed.
NP No.
発現ホスト
CHO Stable Cells
Human
予測N末端
Ser 122
分子量
The mature recombinant human β-NGF consists of 118 amino acids and has a predicted molecular mass of 13.2 kDa. β-NGF exists as a non-disulfide linked homodimer in solution.
バッファー
Lyophilized from sterile 20mM NaAc, 150mM NaCl, pH 5.5
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
配送
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
安定性 & 保存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
再構成
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

NGF Protein, Human, Recombinant: 画像

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.2-2 ng/ml.

NGF Protein, Human, Recombinant: 別名

Beta-NGF Protein, Human; HSAN5 Protein, Human; NGFB Protein, Human

NGF 背景情報

Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. NGF protein was identified as a large complex consisting of three non-covalently linked subunits, α, β, and γ, among which, the β subunit, called β-NGF (beta-NGF), was demonstrated to exhibits the growth stimulating activity of NGF protein. NGFB/beta-NGF gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. NGF protein acts via at least two receptors on the surface of cells (TrkA and p75 receptors) to regulate neuronal survival, promote neurite outgrowth, and up-regulate certain neuronal functions such as mediation of pain and inflammation. In addition, previous studies indicated that NGF may also have an important role in the regulation of the immune system.
完全な名称
nerve growth factor (beta polypeptide)
参考文献
  • Castellanos MR, et al. (2003) Evaluation of the neurorestorative effects of the murine beta-nerve growth factor infusions in old rat with cognitive deficit. Biochem Biophys Res Commun. 312(4): 867-72.
  • Wang TH, et al. (2008) Effects of pcDNA3-beta-NGF gene-modified BMSC on the rat model of Parkinson's disease. J Mol Neurosci. 35(2): 161-9.
  • Perrard MH, et al. (2009) Redundancy of the effect of TGFbeta1 and beta-NGF on the second meiotic division of rat spermatocytes. Microsc Res Tech. 72(8): 596-602.
  • β-Nerve growth factor is a major component of alpaca seminal plasma and induces ovulation in female alpacas
    Author
    Kershaw-Young, CM;Druart, X;Vaughan, J;Maxwell, WM;
    Year
    2012
    Journal
    Reprod. Fertil. Dev.
  • Clinical significance of nerve growth factor and tropomyosin-receptor-kinase signaling pathway in intrahepatic cholangiocarcinoma
    Author
    Yang, XQ;Xu, YF;Guo, S;Liu, Y;Ning, SL;Lu, XF;Yang, H;Chen, YX;
    Year
    2014
    Journal
    World J. Gastroenterol.
    Application
    cell-based
  • Conformational Analysis of Large and Highly Disulfide-Stabilized Proteins by Integrating Online Electrochemical Reduction into an Optimized H/D Exchange Mass Spectrometry Workflow
    Author
    Trabjerg, E;Jakobsen, RU;Mysling, S;Christensen, S;Jørgensen, TJ;Rand, KD;
    Year
    2015
    Journal
    Anal. Chem.
  • Investigating the conformational response of the Sortilin receptor upon binding endogenous peptide- and protein ligands by HDX-MS
    Author
    Trabjerg, E;Abu-Asad, N;Wan, Z;Kartberg, F;Christensen, S;Rand, K;
    Year
    2018
    Journal
    bioRxiv
    Application
    binding
  • Histone deacetylase 6 inhibition restores autophagic flux to promote functional recovery after spinal cord injury
    Author
    Zheng, Z;Zhou, Y;Ye, L;Lu, Q;Zhang, K;Zhang, J;Xie, L;Wu, Y;Xu, K;Zhang, H;Xiao, J;
    Year
    2019
    Journal
    Exp. Neurol.
    Application
    cell-based
  • Nerve growth factor-induced migration in oral and salivary gland tumour cells utilizes the PI3K/Akt signalling pathway: is there a link to perineural invasion?
    Author
    Alkhadar, H;Macluskey, M;White, S;Ellis, I;
    Year
    2019
    Journal
    J. Oral Pathol. Med.
    Application
    cell-based
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