p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag tag

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p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag tag: 製品情報

遺伝子
Mouse
NCBI 参考シーケンス番号
遺伝子の長さ
519 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Mouse cyclin-dependent kinase inhibitor 1A (P21) with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI(6kb+0.52kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

p21/WAF1/CDKN1A cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag tag: 検証済の画像

The plasmid was transfected into 293FT adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

p21/WAF1/CDKN1A cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag tag: 別名

CAP20 cDNA ORF Clone, Mouse; CDKI cDNA ORF Clone, Mouse; Cdkn1 cDNA ORF Clone, Mouse; CIP1 cDNA ORF Clone, Mouse; mda6 cDNA ORF Clone, Mouse; P21 cDNA ORF Clone, Mouse; p21Cip1 cDNA ORF Clone, Mouse; p21WAF cDNA ORF Clone, Mouse; SDI1 cDNA ORF Clone, Mouse; Waf1 cDNA ORF Clone, Mouse
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