Vesicular stomatitis Indiana virus (VSIV) (strain San Juan) glycoprotein / GP Insect Cell Lysate (WB positive control)

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Vesicular stomatitis Indiana virus (VSIV) (strain San Juan) glycoprotein / GP Insect Cell Lysate (WB positive control): 製品の情報

製品の説明
This VSIV glycoprotein/VSIV-GP overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of glycoprotein/VSIV-GP protein (Cat: 40570-V08B) from the overexpression lysate was verified.
発現ホスト
Baculovirus-Insect Cells
VSIV
タンパク質構築情報
A DNA sequence encoding the Vesicular stomatitis Indiana virus (VSIV) (strain San Juan) G (NP_041715.1) (Met1-Ser467) was expressed with a polyhistidine tag at the C-terminus.
分子量
The recombinant Vesicular stomatitis Indiana virus (VSIV) (strain San Juan) G consists of 462 amino acids and predicts a molecular mass of 51.9 kDa.

Vesicular stomatitis Indiana virus (VSIV) (strain San Juan) glycoprotein / GP Insect Cell Lysate (WB positive control): 用法

調製方法
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
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