Mouse FGL1 Baculovirus-Insect Overexpression Lysate

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Mouse FGL1 Baculovirus-Insect Overexpression Lysate: 製品の情報

製品の説明
This Mouse FGL1 overexpression lysate was created in Baculovirus-Insect Cells and intented for use as a Western blot (WB) positive control. Purification of FGL1 protein (Cat: 5A2001-M08B) from the overexpression lysate was verified.
発現ホスト
Baculovirus-Insect Cells
Mouse
タンパク質構築情報
A DNA sequence encoding the mouse FGL1 (NP_663569.2) (Met1-Ile314) was expressed with a polyhistidine tag at the C-terminus.
分子量
The recombinant mouse FGL1 consists of 303 amino acids and predicts a molecular mass of 35.5 kDa.

Mouse FGL1 Baculovirus-Insect Overexpression Lysate: 用法

調製方法
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min. 3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Mouse FGL1 Baculovirus-Insect Overexpression Lysate: 別名

Mouse Mfire1 Overexpression Lysate

FGL1 背景情報

完全な名称
fibrinogen-like 1
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