Human SLITRK1 HEK293 Overexpression Lysate

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Human SLITRK1 HEK293 Overexpression Lysate: 製品の情報

製品の説明
This Human SLITRK1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLITRK1 protein (Cat: 10340-H03H) from the overexpression lysate was verified.
発現ホスト
HEK293 Cells
Human
タンパク質構築情報
A DNA sequence encoding the extracellular domain (Met 1-Ser 616) of human SLITRK1 (NP_443142.1) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human SLITRK1/Fc chimera is a disulfide-linked homodimeric protein after removal of the signal peptide. The reduced monomer consists of 846 amino acids and predicts a molecular mass of 95 kDa. As a result of glycosylation, the apparent molecular mass of rhSLITRK1/Fc monomer is approximately 130-150 kDa in SDS-PAGE under reducing conditions.

Human SLITRK1 HEK293 Overexpression Lysate: 用法

調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human SLITRK1 HEK293 Overexpression Lysate: 別名

Human FLJ54428 Overexpression Lysate; Human KIAA0918 Overexpression Lysate; Human KIAA1910 Overexpression Lysate; Human LRRC12 Overexpression Lysate; Human RP11-395N17.1 Overexpression Lysate; Human SLITRK1 Overexpression Lysate; Human TTM Overexpression Lysate

SLITRK1 背景情報

SLITRK1 (Slit and Trk-like family member 1) is a integral membrane protein belonging to the SLITRK family consists of at least 6 members (SLITRK1-6). They are named and characterized by the presence of two leucine-rich repeats (LRRs) in the extracellular domain similar to those found in a secreted axonal growth-controlling protein, Slit, as well as a C-terminal domain with homology to Trk neurotrophin tyrosine kinase receptors. Expression of SLITRKs are highly restricted to neural tissues, and are identified as the neuronal components modulating the neurite outgrowth. More specifically, SLITRK1 expression is found in the mature neurons of the cerebrum, thalamus and hippocampus, and induces unipolar neurites in cultured neuronal cells. Human SLITRK1 is a 696 amino acid precursor protein, and one truncating frameshift mutation (448 aa) has been linked to Tourette's syndrome, a genetically influenced developmental neuropsychiatric disorder characterized by chronic vocal and motor tics. In addition, all SLITRK genes are differentially expressed in brain tumors, such as astrocytoma, oligodendroglioma, glioblastoma, and are suggested to be useful molecular indicators of brain tumor properties.
完全な名称
SLIT and NTRK like family member 1
参考文献

    1. Aruga, J. and Mikoshiba, K. 2003, Mol. Cell. Neurosci. 24: 117-129.

    2. Aruga, J. et al., 2003, Gene. 315: 87-94.

    3. Abelson, J.F. et al., 2005, Science. 310: 317-320.

    4. Grados, M.A. and Walkup. J.T. 2006, Trends. Genet. 22: 291-293.

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