Human PLGF/PGF HEK293 Overexpression Lysate: 製品の情報
This Human PLGF/PGF overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PLGF/PGF protein (Cat: 10274-H05H) from the overexpression lysate was verified.
A DNA sequence encoding the human PGF (NP_002623.2) (Met1-Arg170) was expressed with the Fc region of mouse IgG1 at the C-terminus.
The recombinant human PGF consists 386 amino acids and predicts a molecular mass of 43.7 kDa.
Human PLGF/PGF HEK293 Overexpression Lysate: 用法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.