Human PDGFRB HEK293 Overexpression Lysate
Cat: 10514-HCCHL
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Human PDGFRB HEK293 Overexpression Lysate: 製品の情報
製品の説明
This Human PDGFRB overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of PDGFRB protein (Cat: 10514-HCCH) from the overexpression lysate was verified.
発現ホスト
HEK293 Cells
種
Human
タンパク質構築情報
A DNA sequence encoding the extracellular domain of human PDGFRβ (NP_002600.1) (Met 1-Lys 531) was expressed with six amino acids (ENLYFQ) at the C-terminus.
分子量
The recombinant human PDGFRβ consists of 506 amino acids and predicts a molecular mass of 57.1 KDa. It migrates as an approximately 92-116 KDa band in SDS-PAGE under reducing conditions.
Human PDGFRB HEK293 Overexpression Lysate: 用法
調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Optimal dilutions/concentrations should be determined by the end user.
Human PDGFRB HEK293 Overexpression Lysate: 別名
Human CD140B Overexpression Lysate; Human IBGC4 Overexpression Lysate; Human IMF1 Overexpression Lysate; Human JTK12 Overexpression Lysate; Human KOGS Overexpression Lysate; Human PDGFR Overexpression Lysate; Human PDGFR-1 Overexpression Lysate; Human PDGFR1 Overexpression Lysate; Human PENTT Overexpression Lysate
PDGFRB 背景情報
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.
完全な名称
platelet-derived growth factor receptor, beta polypeptide
参考文献
- Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
- Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
- Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
- Suzuki S, et al. (2010) Clinicopathological significance of platelet-derived growth factor (PDGF)-B and vascular endothelial growth factor-A expression, PDGF receptor- phosphorylation, and microvessel density in gastric cancer. BMC cancer. 10: 659.
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