Human PCTAIRE1 Baculovirus-Insect cells Overexpression Lysate

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Human PCTAIRE1 Baculovirus-Insect cells Overexpression Lysate: 製品の情報

製品の説明
This Human PCTAIRE1 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of PCTAIRE1 protein (Cat: 10631-H09B) from the overexpression lysate was verified.
発現ホスト
Baculovirus-Insect cells
Human
タンパク質構築情報
A DNA sequence encoding the human PCTK1 (Q00536-1) (Met 1-Phe 496) was fused with the GST tag at the N-terminus.
分子量
The recombinant human PCTK1/GST chimera consists of 720 amino acids and has a predicted molecular mass of 82 kDa. It migrates as an approximately 73 kDa band in SDS-PAGE under reducing conditions.

Human PCTAIRE1 Baculovirus-Insect cells Overexpression Lysate: 用法

調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human PCTAIRE1 Baculovirus-Insect cells Overexpression Lysate: 別名

Human PCTAIRE Overexpression Lysate; Human PCTAIRE1 Overexpression Lysate; Human PCTGAIRE Overexpression Lysate; Human PCTK1 Overexpression Lysate

PCTAIRE1 背景情報

完全な名称
cyclin-dependent kinase 16
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