Human LAMP3/CD208 HEK293 Overexpression Lysate: 製品の情報
This Human LAMP3/CD208 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LAMP3/CD208 protein (Cat: 10527-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the mature form of human LAMP3 (NP_055213.2) (Lys 28-Thr 381) with a carboxy-terminal polyhistidine tag was expressed.
The secreted recombinant human LAMP3 consists of 365 amino acids with the predicted molecular mass of 39 kDa. As a result of glycosylation, rh LAMP3 migrates as an approximately 70-90 kDa band in SDS-PAGE under reducing conditions.
Human LAMP3/CD208 HEK293 Overexpression Lysate: 用法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human LAMP3/CD208 HEK293 Overexpression Lysate: 別名
Human CD208 Overexpression Lysate; Human DC LAMP Overexpression Lysate; Human DC-LAMP Overexpression Lysate; Human DCLAMP Overexpression Lysate; Human LAMP Overexpression Lysate; Human LAMP-3 Overexpression Lysate; Human TSC403 Overexpression Lysate
Dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD28, also known as LAMP3, is a member of the lysosomal associated membrane protein (LAMP) family, which is specifically expressed by human dendritic cells (DCs) upon activation and therefore serves as marker of human DC maturation. Confocal and immunoelectron microscopy showed that mouse DC-LAMP protein co-localizes with lbm18, a specific marker for the limiting membrane of lamellar bodies that contain surfactant protein B. The present study demonstrates that DC-LAMP is constitutively expressed by mouse, sheep, and human type II pneumocytes. DC-LAMP is constitutively expressed in normal type II pneumocytes. DC-LAMP is detected first in activated human DC within MHC class II molecules-containing compartments just before the translocation of MHC class II-peptide complexes to the cell surface, suggesting a possible involvement in this process. Furthermore, overexpression of LAMP3 is actively involved in tumor invasion through increased migration into lymph-vascular spaces.
lysosomal associated membrane protein 3
Salaun B, et al. (2003) Cloning and characterization of the mouse homologue of the human dendritic cell maturation marker CD208/DC-LAMP. Eur J Immunol. 33(9): 2619-29.
Salaun B, et al. (2004) CD208/dendritic cell-lysosomal associated membrane protein is a marker of normal and transformed type II pneumocytes. Am J Pathol. 164(3): 861-71.
Ishigami S, et al. (2010) Prognostic value of CD208-positive cell infiltration in gastric cancer. Cancer Immunol Immunother. 59(3): 389-95.