Human JAM-A/Junctional Adhesion Molecule A HEK293 Overexpression Lysate: 製品の情報
This Human JAM-A/Junctional Adhesion Molecule A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of JAM-A/Junctional Adhesion Molecule A protein (Cat: 10198-H02H) from the overexpression lysate was verified.
A DNA sequence encoding the extracellular domain (Met 1-Ala 242) of human JAM-A (NP_058642.1) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.
The mature recombinant human JAM-A/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer comprises 453 amino acids and has a calculated molecular mass of 50 kDa. As a result of glycosylation, the rh JAM-A/Fc monomer migrates as an approximately 61 kDa protein in SDS-PAGE under reducing conditions.
Human JAM-A/Junctional Adhesion Molecule A HEK293 Overexpression Lysate: 用法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human JAM-A/Junctional Adhesion Molecule A HEK293 Overexpression Lysate: 別名
Human CD321 Overexpression Lysate; Human JAM Overexpression Lysate; Human JAM1 Overexpression Lysate; Human JAMA Overexpression Lysate; Human JCAM Overexpression Lysate; Human KAT Overexpression Lysate; Human PAM-1 Overexpression Lysate
JAM-A/Junctional Adhesion Molecule A 背景情報
Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.
Guglielmi KM, et al. (2007) Reovirus binding determinants in junctional adhesion molecule-A. J Biol Chem. 282(24): 17930-40.
Yeung D, et al. (2008) Decreased junctional adhesion molecule-A expression during blood-brain barrier breakdown. Acta Neuropathol. 115(6): 635-42.
Ong KL, et al. (2009) Elevated plasma level of soluble F11 receptor/junctional adhesion molecule-A (F11R/JAM-A) in hypertension. Am J Hypertens. 22(5): 500-5.