Human Interferon alpha-B HEK293 Overexpression Lysate: 製品の情報
This Human Interferon alpha-B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Interferon alpha-B protein (Cat: 10347-HNCH) from the overexpression lysate was verified.
A DNA sequence encoding the human IFNA8 (NP_002161.2)(Cys24-Glu189) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus.
The secreted recombinant human IFNA8 consists of 168 amino acids and predicts a molecular mass of 19.6 KDa. The apparent molecular mass of the protein is approximately 26 KDa in SDS-PAGE under reducing conditions due to glycosylation.
Human Interferon alpha-B HEK293 Overexpression Lysate: 用法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human Interferon alpha-B HEK293 Overexpression Lysate: 別名
Human IFN-alphaB Overexpression Lysate
Interferon alpha-B 背景情報
Interferon alpha-B, also known as IFNA8, belongs to the alpha/beta interferon family. Interferons are proteins made and released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites, or tumor cells. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They also allow for communication between cells to trigger the protective defenses of the immune system that eradicate pathogens or tumors. Interferons also activate immune cells, such as natural killer cells and macrophages. They increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes. They also increase the ability of uninfected host cells to resist new infections by virus. Certain symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. Produced by macrophages, IFN-alpha has antiviral activities.
interferon, alpha 8
Henco K. et al., 1985, J Mol Biol. 185 (2): 227-60.
Goeddel DV. et al., 1981, Nature. 290 (5801): 20-6.
Yelverton E. et al., 1981, Nucleic Acids Res. 9 (3): 731-41.
Kempaiah P. et al., 2012, Hum Genet. 131 (8): 1375-91.