Human interferon-alpha/IFNA5 HEK293 Overexpression Lysate

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Human interferon-alpha/IFNA5 HEK293 Overexpression Lysate: 製品の情報

製品の説明
This Human interferon-alpha/IFNA5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of interferon-alpha/IFNA5 protein (Cat: 10342-H01H) from the overexpression lysate was verified.
発現ホスト
HEK293 Cells
Human
タンパク質構築情報
A DNA sequence encoding the human IFNα G (NP_002160.1) (Leu 22-Glu 189) was fused with the Fc region of human IgG1 at the N-terminus.
分子量
The recombinant human IFNα G/Fc chimera is a disulfide-linked homodimer. The reduced monomer comprises 405 amino acids with a predicted molecular mass of 46.3 kDa. As a result of glycosylation, rh IFNα G/Fc monomer migrates as an approximately 48-50 kDa band in SDS-PAGE under reducing conditions.

Human interferon-alpha/IFNA5 HEK293 Overexpression Lysate: 用法

調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human interferon-alpha/IFNA5 HEK293 Overexpression Lysate: 別名

Human IFN-alpha Overexpression Lysate; Human IFN-alpha-5 Overexpression Lysate; Human IFN-alphaG Overexpression Lysate; Human INA5 Overexpression Lysate; Human INFA5 Overexpression Lysate; Human leIF G Overexpression Lysate; Human RP11-380P16.5 Overexpression Lysate

interferon-alpha/IFNA5 背景情報

Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.
完全な名称
interferon, alpha 5
参考文献
  • Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9.
  • Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65.
  • Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.
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