Human IL1RAPL1 HEK293 Overexpression Lysate

Price:
Size:
Number:

Human IL1RAPL1 HEK293 Overexpression Lysate: 製品の情報

製品の説明
This Human IL1RAPL1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL1RAPL1 protein (Cat: 10177-H02H) from the overexpression lysate was verified.
発現ホスト
HEK293 Cells
Human
タンパク質構築情報
A DNA sequence encoding the human IL1R8 (NP_055086.1) extracellular domain (Met 1-Leu 354) was fused with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human IL1R8/Fc is a disulfide-linked homodimer. The reduced monomer consists of 574 amino acids and has a predicted molecular mass of 65 kDa. As a result of glycosylation, the rh IL1R8/Fc monomer migrates with an apparent molecular mass of 80-85 kDa in SDS-PAGE under reducing conditions.

Human IL1RAPL1 HEK293 Overexpression Lysate: 用法

調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human IL1RAPL1 HEK293 Overexpression Lysate: 別名

Human IL1R8 Overexpression Lysate; Human IL1RAPL Overexpression Lysate; Human MRX10 Overexpression Lysate; Human MRX21 Overexpression Lysate; Human MRX34 Overexpression Lysate; Human OPHN4 Overexpression Lysate; Human TIGIRR-2 Overexpression Lysate

IL1RAPL1 背景情報

Interleukin-1 receptor accessory protein-like 1 (IL1RAPL1) is a member of interleukin-1 receptor family. The protein structurally comprises three extracellular immunoglobulin domains, which presumably mediate binding of an as yet unidentified ligand, a transmembrane region, and an intracellular domain, which is likely to enable signalling via the NFkB pathway. The means of signalling is almost certain to be identical to that used by the IL1R family and the more distally related Toll protein. L1RAPL1 protein physically interacts via its 15 aa C-terminal domain with neuronal calcium sensor-1 (NCS-1), a protein widely expressed in neurons and the related chromaffin and PC12 cells. IL1RAPL1 is an integral membrane protein responsible for a nonsyndromic form of mental retardation (MR). It is suggested to affect human cognitive ability to some extent, especially the memory and concentration capability.
完全な名称
interleukin 1 receptor accessory protein-like 1
参考文献
  • Frdric Gambino, et al. (2007) IL1-receptor accessory protein-like 1 (IL1RAPL1), a protein involved in cognitive functions, regulates N-type Ca2+-channel and neurite elongation. Proc Natl Acad Sci. 104 (21): 9063-8.
  • Wheway JM, et al. (2003) A complex deletion-inversion-deletion event results in a chimeric IL1RAPL1-dystrophin transcript and a contiguous gene deletion syndrome. J Med Genet. 40: 127-131.
カートに追加しました カートに追加しませんでした カートを更新しています。少々お待ちください U.S.A.