Human IL-6R HEK293 Overexpression Lysate
Cat: 10398-H02HL
Cat: 10398-H02HL
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Human IL-6R HEK293 Overexpression Lysate: 製品の情報
製品の説明
This Human IL-6R overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of IL-6R protein (Cat: 10398-H02H) from the overexpression lysate was verified.
発現ホスト
HEK293 Cells
種
Human
タンパク質構築情報
A DNA sequence encoding the human IL6R (NP_000556.1) (Met1-Pro365) was expressed with the Fc region of human IgG1 at the C-terminus.
分子量
The recombinant human IL6R consists of 464 amino acids and predicts a molecular mass of 52.2 kDa.
Human IL-6R HEK293 Overexpression Lysate: 用法
調製方法
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
3. Store the lyophilized cell lysate at 4℃. After re-dissolution, recommend to aliquot it into smaller quantities and store at -80℃.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Optimal dilutions/concentrations should be determined by the end user.
Human IL-6R HEK293 Overexpression Lysate: 別名
Human CD126 Overexpression Lysate; Human gp80 Overexpression Lysate; Human IL-6R Overexpression Lysate; Human IL-6R-1 Overexpression Lysate; Human IL-6RA Overexpression Lysate; Human IL6Q Overexpression Lysate; Human IL6RA Overexpression Lysate; Human IL6RQ Overexpression Lysate
IL-6R 背景情報
Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp13. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp13 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.
完全な名称
interleukin 6 receptor
参考文献
- Barill S, et al. (2000) The role of interleukin-6 and interleukin-6/interleukin-6 receptor-alpha complex in the pathogenesis of multiple myeloma. Eur Cytokine Netw. 11(4): 546-51.
- Kang KW, et al. (2007) Novel role of IL-6/SIL-6R signaling in the expression of inducible nitric oxide synthase (iNOS) in murine B16, metastatic melanoma clone F10.9, cells. Free Radic Biol Med. 42(2): 215-27.
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