Human HER3/ERBB3 HEK293 Overexpression Lysate

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Human HER3/ERBB3 HEK293 Overexpression Lysate: 製品の情報

製品の説明
This Human HER3/ERBB3 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of HER3/ERBB3 protein (Cat: 10201-H05H) from the overexpression lysate was verified.
発現ホスト
HEK293 Cells
Human
タンパク質構築情報
A DNA sequence encoding the human ErbB3 (NP_001973.2) (Met1-Thr643)was fused with Fc region of mouse IgG at the C-terminus.
分子量
The recombinant human ErbB3/mFc is a disulfide-linked homodimer. The reduced monomer comprises 858 amino acids and has a predicted molecular mass of 95 kDa. The apparent molecular mass of the protein is approximately 119-129 in SDS-PAGE under reducing conditions.

Human HER3/ERBB3 HEK293 Overexpression Lysate: 用法

調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human HER3/ERBB3 HEK293 Overexpression Lysate: 別名

Human c-erbB-3 Overexpression Lysate; Human c-erbB3 Overexpression Lysate; Human EEBB3 Overexpression Lysate; Human ErbB-3 Overexpression Lysate; Human erbB3-S Overexpression Lysate; Human HER3 Overexpression Lysate; Human LCCS2 Overexpression Lysate; Human MDA-BF-1 Overexpression Lysate; Human p180-ErbB3 Overexpression Lysate; Human p45-sErbB3 Overexpression Lysate; Human p85-sErbB3 Overexpression Lysate

HER3/ERBB3 背景情報

ErbB3, also known as Her3(human epidermal growth factor receptor3), is a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound glycoprotein has a neuregulin binding domain but has not an active kinase domain., and therefore can not mediate the intracellular signal transduction through protein phosphorylation. However, its heterodimer with ErbB2 or other EGFR members responsible for tyrosine phosphorylation forms a receptor complex with high affinity, and initiates the related pathway which lead to cell proliferation or differentiation. ErbB3 has been shown to implicated in numerous cancers, including prostate, bladder, and breast tumors. This protein has different isoforms derived from alternative splicing variants, and among which, the secreted isoform lacking the intermembrane region modulates the activity of membrane-bound form.
完全な名称
erb-b2 receptor tyrosine kinase 3
参考文献
  • Kraus M.H., et al.,(1989), Isolation and characterization of ERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Natl. Acad. Sci. U.S.A. 86:9193-9197.
  • Plowman G.D., et al., (1990), Molecular cloning and expression of an additional epidermal growth factor receptor-related gene.Proc. Natl. Acad. Sci. U.S.A. 87:4905-4909.
  • Katoh M., et al.,(1993), c-erbB3 gene encodes secreted as well as transmembrane receptor tyrosine kinase.Biochem. Biophys. Res. Commun. 192:1189-1197.
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