Human HAI-2/SPINT2 HEK293 Overexpression Lysate: 製品の情報
This Human HAI-2/SPINT2 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of HAI-2/SPINT2 protein (Cat: 10324-H08H) from the overexpression lysate was verified.
A DNA sequence encoding the human SPINT2 (NP_066925.1) (Met1-Lys197) was expressed with a polyhistidine tag at the C-terminus.
The recombinant human SPINT2 consists 181 amino acids and predicts a molecular mass of 20.6 kDa.
Human HAI-2/SPINT2 HEK293 Overexpression Lysate: 用法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB) Optimal dilutions/concentrations should be determined by the end user.
Human HAI-2/SPINT2 HEK293 Overexpression Lysate: 別名
Human DIAR3 Overexpression Lysate; Human HAI-2 Overexpression Lysate; Human HAI2 Overexpression Lysate; Human Kop Overexpression Lysate; Human PB Overexpression Lysate
Choanal (CA) and gastrointestinal atresias (GA) are an important feature of syndromic congenital sodium diarrhea (sCSD), a disorder recently associated with mutations in the gene for serine protease inhibitor type 2 (SPINT2). The SPINT2 gene is epigenetically silenced or downregulated in human cancers, altering the balance of HGF activation/inhibition ratio, which contributes to cancer development and progression. SPINT2 is a tumor suppressor gene that inhibits proteases implicated in cancer progression, like HGFA, hepsin and matriptase. Loss of SPINT2 expression in tumors has been associated with gene promoter hypermethylation. SPINT2 (serine peptidase inhibitor Kunitz type 2), a proteolytic inhibitor of hepatocyte growth factor activator (HGFA), has a significant role in the suppression of the HGF-MET pathway and malignant melanoma progression.