Human DKK1 HEK293 Overexpression Lysate

Human DKK1 HEK293 Overexpression Lysate: 製品の情報

This Human DKK1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of DKK1 protein (Cat: 10170-H08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the human DKK1 precursor (NP_036374.1) (Met 2-His 266) was expressed with a C-terminal polyhistidine tag.
The recombinant human DKK1 protein consists of 246 amino acids and has a calculated molecular mass of 27.22 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh DKK1 is approximately 42.9 kDa due to glycosylation.

Human DKK1 HEK293 Overexpression Lysate: 用法

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Human DKK1 HEK293 Overexpression Lysate: 別名

Human DKK-1 Overexpression Lysate; Human SK Overexpression Lysate

DKK1 背景情報

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.
dickkopf WNT signaling pathway inhibitor 1
Related Pathways
  • Canonical Wnt Pathway
    Canonical Wnt Pathway
  • Horwitz EM. (2004) Dkk-1-mediated expansion of adult stem cells. Trends Biotechnol. 22(8): 386-8.
  • Jiang T, et al. (2009) Clinical significance of serum DKK-1 in patients with gynecological cancer. Int J Gynecol Cancer. 19(7): 1177-81.
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