Human alk5 Baculovirus-Insect cells Overexpression Lysate
Cat: 10459-H20BL
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Human alk5 Baculovirus-Insect cells Overexpression Lysate: 製品の情報
製品の説明
This Human alk5 overexpression lysate was created in Baculovirus-Insect cells and intented for use as a Western blot (WB) positive control. Purification of alk5 protein (Cat: 10459-H20B) from the overexpression lysate was verified.
発現ホスト
Baculovirus-Insect cells
種
Human
タンパク質構築情報
A DNA sequence encoding the human ALK5 (P36897-1) (Thr200-Mey503) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
分子量
The recombinant human ALK5/GST chimera consists of 541 amino acids and has a calculated molecular mass of 62.6 kDa. The recombinant protein migrates approximately 57 kDa band in SDS-PAGE under reducing conditions.
Human alk5 Baculovirus-Insect cells Overexpression Lysate: 用法
調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.
2. Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.
Optimal dilutions/concentrations should be determined by the end user.
Human alk5 Baculovirus-Insect cells Overexpression Lysate: 別名
Human AAT5 Overexpression Lysate; Human ACVRLK4 Overexpression Lysate; Human ALK-5 Overexpression Lysate; Human ALK5 Overexpression Lysate; Human ESS1 Overexpression Lysate; Human LDS1 Overexpression Lysate; Human LDS1A Overexpression Lysate; Human LDS2A Overexpression Lysate; Human MSSE Overexpression Lysate; Human SKR4 Overexpression Lysate; Human tbetaR-I Overexpression Lysate; Human TGFR-1 Overexpression Lysate
alk5 背景情報
Transforming growth factor, beta receptor I, also known as Transforming growth factor-beta receptor type I , Serine / threonine-protein kinase receptor R4, Activin receptor-like kinase 5, SKR4, ALK-5, and TGFBR1, is a single-pass type I membrane protein that belongs to the protein kinase superfamily and TGFB receptor subfamily. TGFBR1 / ALK-5 is found in all tissues examined. It is most abundant in placenta and least abundant in brain and heart. TGF-beta functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase. Administration of TGF-beta is able to protect against mammary tumor development in transgenic mouse models in vivo. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers, with the majority of colon and gastric cancers being caused by an inactivating mutation of TGF-beta RII. On ligand binding, TGFBR1 / ALK-5 forms a receptor complex consisting of two type I I and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which auto-phosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta signaling via TGFBR1 / ALK-5 is not required in myocardial cells during mammalian cardiac development, but plays an irreplaceable cell-autonomous role regulating cellular communication, differentiation and proliferation in endocardial and epicardial cells. Defects in TGFBR1 / ALK-5 are the cause of Loeys-Dietz syndrome type 1A (LDS1A), Loeys-Dietz syndrome type 2A (LDS2A), and aortic aneurysm familial thoracic type 5 (AAT5).
完全な名称
transforming growth factor, beta receptor 1
参考文献
- Seki T, et al. (2006) Nonoverlapping expression patterns of ALK1 and ALK5 reveal distinct roles of each receptor in vascular development. Lab Invest. 86(2): 116-29. et al.
- Piek E, et al. (1999) TGF-(beta) type I receptor/ALK-5 and Smad proteins mediate epithelial to mesenchymal transdifferentiation in NMuMG breast epithelial cells. J Cell Sci. 112 (24): 4557-68. et al.
- Dudas M, et al. (2004) Tgf-beta3-induced palatal fusion is mediated by Alk-5/Smad pathway. Dev Biol. 266(1): 96-108.
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