Cynomolgus Interferon alpha-B HEK293 Overexpression Lysate


Cynomolgus Interferon alpha-B HEK293 Overexpression Lysate: 製品の情報

This Cynomolgus Interferon alpha-B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Interferon alpha-B protein (Cat: 90106-C08H) from the overexpression lysate was verified.
HEK293 Cells
A DNA sequence encoding the cynomolgus IFNA8 (G7PS89) (Met1-Glu189) was expressed with a polyhistidine tag at the C-terminus.
The recombinant cynomolgus IFNA8 comprises 177 amino acids and has a calculated molecular mass of 21 KDa. The apparent molecular mass of it is approximately 33 KDa respectively in SDS-PAGE.

Cynomolgus Interferon alpha-B HEK293 Overexpression Lysate: 用法

Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

Interferon alpha-B 背景情報

Interferon alpha-B, also known as IFNA8, belongs to thealpha/beta interferon family. Interferons areproteinsmade and released byhost cellsin response to the presence ofpathogenssuch asviruses,bacteria,parasitesortumorcells. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. They also allow for communication between cells to trigger the protective defenses of theimmune systemthat eradicate pathogens or tumors.Interferons also activate immune cells, such asnatural killer cellsandmacrophages. They increase recognition of infection or tumor cells by up-regulatingantigen presentation toT lymphocytes. They also increase the ability of uninfected host cells to resist new infection by virus. Certain symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. Produced by macrophages, IFN-alpha have antiviral activities.
interferon, alpha 8
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