Human cytomegalovirus (HCMV) Glycoprotein B / gB HEK293 Cell Lysate (WB positive control)

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Human cytomegalovirus (HCMV) Glycoprotein B / gB HEK293 Cell Lysate (WB positive control): 製品の情報

製品の説明
This CMV CMV Glycoprotein B overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of CMV Glycoprotein B protein (Cat: 10202-VCCH1) from the overexpression lysate was verified.
発現ホスト
HEK293 Cells
CMV
タンパク質構築情報
A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') was expressed, fused with five additional amino acids (DDDDK) at the C-terminus.
分子量
The recombinant glycoprotein B of human CMV consists of 813 amino acids and has a predicted molecular mass of 92 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB is approximately 130-140 kDa due to glycosylation.

Human cytomegalovirus (HCMV) Glycoprotein B / gB HEK293 Cell Lysate (WB positive control): 用法

調製方法
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (Bio-Rad protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
溶解バッファー
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
おすすめの用法
1.  Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube. 2.  Re-dissolve the pellet using 200μL pure water and boil for 2-5 min.
バッファー
1 X Sample Buffer (1 X modified RIPA buffer+1 X SDS loading buffer).
安定性 & 保存条件
Store at 4℃ for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80℃ for up to twelve months. Avoid repeated freeze-thaw cycles.
アプリケーション
Western Blot (WB)
Optimal dilutions/concentrations should be determined by the end user.

CMV Glycoprotein B 背景情報

Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
参考文献
  • Lopper M,et al. (2002). Disulfide bond configuration of human cytomegalovirus glycoprotein B. J Virol. 76(12): 6073-82.
  • Isaacson MK, et al. (2009) Human cytomegalovirus glycoprotein B is required for virus entry and cell-to-cell spread but not for virion attachment, assembly, or egress. J Virol. 83(8): 3891-903.
  • Application of Nanotrap technology for high sensitivity measurement of urinary outer surface protein A carboxyl-terminus domain in early stage Lyme borreliosis
    Author
    Magni, R;Espina, BH;Shah, K;Lepene, B;Mayuga, C;Douglas, TA;Espina, V;Rucker, S;Dunlap, R;Petricoin, EF;Kilavos, MF;Poretz, DM;Irwin, GR;Shor, SM;Liotta, LA;Luchini, A;
    Year
    2015
    Journal
    J Transl Med
    Application
    WB positive control
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