Anti-Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Magnetic Beads Immunoprecipitation (IP) Kit

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抗ZIKV NS-1 protein 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-ZIKV NS-1 protein Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-ZIKV NS-1 protein magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗ZIKV NS-1 protein 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-ZIKV NS-1 protein magnetic Beads, conjugated with Anti-ZIKV NS-1 protein antibody, are used for immuneprecipitation (IP) of ZIKV NS-1 protein proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing ZIKV NS-1 protein proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound ZIKV NS-1 protein proteins are dissociated from the magnetic beads using an elution buffer.

抗ZIKV NS-1 protein 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Antibody, Mouse MAb(40544-MM15)
免疫原
Recombinant Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 Protein (Catalog#40544-V07H)
交差反応
Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1
ソース
Monoclonal ZIKV Mouse IgG1
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Zika virus (ZIKV) (strain Zika SPH2015) ZIKV-NS1 ( Catalog#40544-V07H; ALU33341.1; Val796-Leu1157). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

ZIKV NS-1 protein 背景情報

Zika virus NS1 antigen is one of seven non-structural proteins. NS1 is involved in RNA replicaiton. The possible effects of NS1 on hosts include: localization to host cell surface and secreted extracellularly, modelates signalling of innate immune system, has possible damages to platelets and endothelial cells through anti-NS1 antibodies.
参考文献
  • Wong SS-Y, et al., Zika virus infectiondthe next wave after dengue?, Journal of the Formosan Medical Association (2016)
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