Anti-Smad3 Magnetic Beads Immunoprecipitation (IP) Kit

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抗Smad3 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-Smad3 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Smad3 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗Smad3 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-Smad3 magnetic Beads, conjugated with Anti-Smad3 antibody, are used for immuneprecipitation (IP) of Smad3 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Smad3 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Smad3 proteins are dissociated from the magnetic beads using an elution buffer.

抗Smad3 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-Smad3 Antibody, Rabbit Polyclonal(100424-T36)
免疫原
A synthetic peptide corresponding to the center region of the human SMAD3
交差反応
Human Mouse, Rat (Species predicted to react based on 100% sequence homology)
ソース
Polyclonal Human Rabbit IgG
調製
Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human SMAD3, and purified by antigen affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Smad3 Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-HSPC193ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HsT17436ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-JV15-2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-LDS1CALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-LDS3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MADH3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Smad3 背景情報

SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFβ's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.
完全な名称
SMAD family member 3
Related Pathways
  • TGF-beta Signaling
    TGF-beta Signaling
参考文献
  • Tan. et al., 2011, Diabetes. 60 (2): 464-76.
  • Yang X. et al., 1999, Nat Cell Biol. 1 (5): 260-6.
  • Zhu Y. et al., 1998, Cell. 94 (6): 703-14.
  • Feng X. et al., 1996, Nature. 383 (6596): 168-72.
  • Nanosized functional miRNA liposomes and application in the treatment of TNBC by silencing Slug gene
    Author
    Yan, Y;Li, XQ;Duan, JL;Bao, CJ;Cui, YN;Su, ZB;Xu, JR;Luo, Q;Chen, M;Xie, Y;Lu, WL;
    Year
    2019
    Journal
    Int J Nanomedicine
    Application
    IP
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