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Anti-PARP Magnetic Beads Immunoprecipitation (IP) Kit

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抗PARP 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-PARP Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-PARP magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗PARP 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-PARP magnetic Beads, conjugated with Anti-PARP antibody, are used for immuneprecipitation (IP) of PARP proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing PARP proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound PARP proteins are dissociated from the magnetic beads using an elution buffer.

抗PARP 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-PARP Antibody(100984-T46)
免疫原
A synthetic peptide corresponding to the center region of the Human PARP-1 / PARP
交差反応
Human PARP-1 / PARP
ソース
Polyclonal Human Rabbit IgG
調製
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PARP-1 / PARP, and purified by antigen affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-PARP Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-ADPRTALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ADPRT1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ARTD1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-pADPRT-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PARPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PARP-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PPOLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

PARP 背景情報

Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 2-3 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.
完全な名称
poly (ADP-ribose) polymerase 1
参考文献
  • Malanga M. et al., 1998, J Biol Chem. 273: 11839-11843.
  • Ariumi Y. et al., 1999, Oncogene. 18: 4616-4625.
  • Helleday T. et al., 2005, Cell Cycle. 4: 1176-1178.
  • Ahell I. et al., 2008, Nature. 451: 81-85.
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