Anti-p38 Magnetic Beads Immunoprecipitation (IP) Kit

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抗p38 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-p38 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-p38 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗p38 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-p38 magnetic Beads, conjugated with Anti-p38 antibody, are used for immuneprecipitation (IP) of p38 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing p38 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound p38 proteins are dissociated from the magnetic beads using an elution buffer.

抗p38 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-p38 Antibody(10081-T60)
免疫原
Recombinant Human p38 alpha / MAPK14 protein (Catalog#10081-H07B)
交差反応
Human p38 alpha / MAPK14
ソース
Polyclonal Human Rabbit IgG
調製
Produced in rabbits immunized with purified, recombinant Human p38 alpha / MAPK14 (rh p38 alpha / MAPK14; Catalog#10081-H07B; NP_620581.1; Met1-Ser360). p38 alpha / MAPK14 specific IgG was purified by Human p38 alpha / MAPK14 affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-p38 Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-CSBPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CSBP1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CSBP2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CSPB1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-EXIPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-Mxi2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p38ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p38ALPHAALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PRKM14ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PRKM15ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-RKALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SAPK2AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

p38 背景情報

MAPK14 contains 1 protein kinase domain and belongs to the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK14 can be detected in brain, heart, placenta, pancreas and skeletal muscle and it is expressed to a lesser extent in lung, liver and kidney. MAPK14 is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with MAPK14. The substrates of p38 alpha include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of p38 alpha in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. In respond to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide, MAPK14 phosphorylates a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. MAPK14 plays a critical role in the production of some cytokines, for example IL-6. It may play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2.
完全な名称
mitogen-activated protein kinase 14
Related Pathways
  • Non-Canonical Wnt Pathway
    Non-Canonical Wnt Pathway
  • p53 Pathway
    p53 Pathway
  • TGF-beta Signaling
    TGF-beta Signaling
  • TNF Signaling
    TNF Signaling
  • VEGF Signaling Pathway
    VEGF Signaling Pathway
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • P38 MAPK Signaling Pathway
    P38 MAPK Signaling Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
  • IL1 signaling pathway
    IL1 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Luo X, et al. (2011) Study on p38 mitogen activated protein kinase in vascular endothelial cells dysfunction in preeclampsia. Zhonghua Fu Chan Ke Za Zhi. 46(1):36-40.
  • Park CH, et al. (2011) Epidermal growth factor-induced matrix metalloproteinase-1 expression is negatively regulated by p38 MAPK in human skin fibroblasts. J Dermatol Sci. 64(2):134-41.
  • Lee JY, et al. (2011) Curcumin induces EGFR degradation in lung adenocarcinoma and modulates p38 activation in intestine: the versatile adjuvant for gefitinib therapy. PLoS One. 6(8):e23756.
  • Riis JL, et al. (2011) CCL27 expression is regulated by both p38 MAPK and IKKβ signalling pathways. Cytokine. 56(3):699-707.
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