Anti-P38 Delta/MAPK13 Magnetic Beads Immunoprecipitation (IP) Kit

Temporarily not available outside of China.

抗P38 Delta/MAPK13 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-P38 Delta/MAPK13 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-P38 Delta/MAPK13 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗P38 Delta/MAPK13 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-P38 Delta/MAPK13 magnetic Beads, conjugated with Anti-P38 Delta/MAPK13 antibody, are used for immuneprecipitation (IP) of P38 Delta/MAPK13 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing P38 Delta/MAPK13 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound P38 Delta/MAPK13 proteins are dissociated from the magnetic beads using an elution buffer.

抗P38 Delta/MAPK13 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-P38 Delta/MAPK13 Antibody, Rabbit Polyclonal(10747-T52)
免疫原
Recombinant Human p38 delta / MAPK13 Protein (Catalog#10747-H09B)
交差反応
Human p38 delta / MAPK13
ソース
Polyclonal Human Rabbit IgG
調製
Produced in rabbits immunized with purified, recombinant Human p38 delta / MAPK13 (rh p38 delta / MAPK13; Catalog#10747-H09B; O15264; Met1-Leu365). p38 delta / MAPK13 specific IgG was purified by Human p38 delta / MAPK13 affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-P38 Delta/MAPK13 Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-MAPK-13ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MAPK13ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-p38deltaALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PRKM13ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SAPK4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

P38 Delta/MAPK13 背景情報

The p38 family of mitogen-activated protein kinases (MAPK) includes p38 alpha (SAPK2a, CSBP), p38 beta (SAPK2b), p38 delta (SAPK4), and p38 gamma (SAPK3/ERK6). p38 alpha and p38 beta are widely expressed p38 isoforms that are involved in the regulation of cell proliferation, differentiation, development, and stress response. p38 delta, also known as MAPK13, is a regulator of differentiation-dependent gene expression in keratinocytes and has been a regulator of surface epithelial differentiation and apoptosis. p38 delta protein is upregulated in Cholangiocarcinoma (CC) relative to hepatocellular carcinoma (HCC) and normal biliary tract tissues. p38 delta is important for motility and invasion of CC cells, suggesting that p38 delta may play an important role in CC metastasis. p38 delta is expressed in the epidermis, suggesting a role for p38 delta in regulating differentiation. p38 delta is the major p38 isoform driving suprabasal involucrin gene expression and that p38 delta directly regulates ERK1/2 activity via the formation of a p38 delta-ERK1/2 complex. Recent emerging evidence suggests that the p38 stress MAPK pathway may function as a tumor suppressor through regulating Ras-dependent and -independent proliferation, transformation, invasion, and cell death by isoform-specific mechanisms. p38 delta has an important role in promoting cell proliferation and tumor development in the epidermis and may have therapeutic implications for skin cancer.
完全な名称
mitogen-activated protein kinase 13
Related Pathways
  • VEGF Signaling Pathway
    VEGF Signaling Pathway
  • IL1 signaling pathway
    IL1 signaling pathway
  • IL17 signaling pathway
    IL17 signaling pathway
参考文献
  • Efimova T, et al. (2003) A regulatory role for p38 delta MAPK in keratinocyte differentiation. Evidence for p38 delta-ERK1/2 complex formation. J Biol Chem. 278(36): 34277-85.
  • Eckert RL, et al. (2003) p38 Mitogen-activated protein kinases on the body surface--a function for p38 delta. J Invest Dermatol. 120(5): 823-8.
  • Loesch M, et al. (2008) The p38 MAPK stress pathway as a tumor suppressor or more? Front Biosci. 13: 3581-93.
  • Schindler EM, et al. (2009) p38delta Mitogen-activated protein kinase is essential for skin tumor development in mice. Cancer Res. 69(11): 4648-55.
  • Tan FL, et al. (2010) p38delta/MAPK13 as a diagnostic marker for cholangiocarcinoma and its involvement in cell motility and invasion. Int J Cancer. 126(10): 2353-61.
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