Anti-Influenza A H1N1 (Swine Flu 2009) Neuraminidase / NA Magnetic Beads Immunoprecipitation (IP) Kit

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抗Neuraminidase/NA 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-Neuraminidase/NA Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Neuraminidase/NA magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗Neuraminidase/NA 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-Neuraminidase/NA magnetic Beads, conjugated with Anti-Neuraminidase/NA antibody, are used for immuneprecipitation (IP) of Neuraminidase/NA proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Neuraminidase/NA proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Neuraminidase/NA proteins are dissociated from the magnetic beads using an elution buffer.

抗Neuraminidase/NA 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Influenza A H1N1 (Swine Flu 2009) Neuraminidase / NA Antibody, Mouse MAb(11058-MM07)
免疫原
Recombinant H1N1 NA / Neuraminidase protein
交差反応
H1N1 (A/California/04/2009) NA / Neuraminidase
ソース
Monoclonal Influenza Mouse IgG1
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant influenza A virus H1N1 Neuraminidase. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography .
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Neuraminidase/NA 背景情報

Neuraminidases are enzymes that cleave sialic acid groups from glycoproteins. Influenza neuraminidase is a type of neuraminidase found on the surface of influenza viruses that enables the virus to be released from the host cell. Influenza neuraminidase is composed of four identical subunits arranged in a square. It is normally attached to the virus surface through a long protein stalk. The active sites are in a deep depression on the upper surface. They bind to polysaccharide chains and clip off the sugars at the end. The surface of neuraminidase is decorated with several polysaccharide chains that are similar to the polysaccharide chains that decorate our own cell surface proteins. Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Neuraminidase, on the other hand, cleaves the HA-sialic acid bondage from the newly formed virions and the host cell receptors during budding. Neuraminidase thus is described as a receptor-destroying enzyme which facilitates virus release and efficient spread of the progeny virus from cell to cell. Influenza antibody and influenza antibodies are very important research tools for influenza diagnosis, influenza vaccine development, and anti-influenza virus therapy development. Monoclonal or polyclonal antibody can be raised with protein based antigen or peptide based antigen. Antibody raised with protein based antigen could have better specificity and/or binding affinity than antibody raised with peptide based antigen, but cost associated with the recombinant protein antigen is usually higher. Anti influenza virus hemagglutinin (HA) monoclonal antibody or polyclonal antibody can be used for ELISA assay, western blotting detection, Immunohistochemistry (IHC), flow cytometry, neutralization assay, hemagglutinin inhibition assay, and early diagnosis of influenza viral infection. Sino Biological has developed state-of-the-art monoclonal antibody development technology platforms: mouse monoclonal antibody and rabbit monoclonal antibody. Our rabbit monoclonal antibody platform is one of a kind and offers some unique advantages over mouse monoclonal antibodies, such as high affinity, low cross-reactivity with rabbit polyclonal antibodies.
参考文献
  • Sardet C., et al.,(1989), Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter. Cell 56:271-280.
  • Sardet C., et al., (1990), Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.Science 247:723-726.
  • Tse C.-M., et al.,(1991), Molecular cloning and expression of a cDNA encoding the rabbit ileal villus cell basolateral membrane Na+/H+ exchanger.EMBO J. 10:1957-1967.
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