Anti-HMGB1 Magnetic Beads Immunoprecipitation (IP) Kit

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抗HMGB1 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-HMGB1 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-HMGB1 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗HMGB1 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-HMGB1 magnetic Beads, conjugated with Anti-HMGB1 antibody, are used for immuneprecipitation (IP) of HMGB1 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing HMGB1 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound HMGB1 proteins are dissociated from the magnetic beads using an elution buffer.

抗HMGB1 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-HMGB1 Antibody, Rabbit Polyclonal(100930-T46)
免疫原
A synthetic peptide corresponding to the C-terminus of the Human HMGB1/HMG1
交差反応
Human, Mouse, Rat
ソース
Polyclonal Human Rabbit IgG
調製
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human HMGB1/HMG1 , and purified by antigen affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-HMGB1 Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-HMG-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HMG1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HMG3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-SBP-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

HMGB1 背景情報

High-mobility group box 1 protein (HMGB1), also known as HMG-1 or amphoterin previously, is a member of the HMGB family consisting of three members, HMGB1, HMGB2, and HMGB3. HMGB1 is a DNA-binding nuclear protein, released actively following cytokine stimulation as well as passively during cell death. It is the prototypic damage-associated molecular pattern (DAMP) molecule and has been implicated in several inflammatory disorders. HMGB1 signals via the receptor for advanced glycation end-product (RAGE) and members of the toll-like receptor (TLR) family. The most prominent HMGB1 protein and mRNA expression arthritis are present in pannus regions, where synovial tissue invades articular cartilage and bone. HMGB1 promotes the activity of proteolytic enzymes, and osteoclasts need HMGB1 for functional maturation. As a non-histone nuclear protein, HMGB1 has a dual function. Inside the cell, HMGB1 binds DNA, regulating transcription, and determining chromosomal architecture. Outside the cell, HMGB1 can serve as an alarmin to activate the innate system and mediate a wide range of physiological and pathological responses. Extracellular HMGB1 represents an optimal " necrotic marker" selected by the innate immune system to recognize tissue damage and initiate reparative responses. However, extracellular HMGB1 also acts as a potent pro-inflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. HMGB1 has been successfully therapeutically targeted in multiple preclinical models of infectious and sterile diseases including arthritis. As shown in studies on patients as well as animal models, HMGB1 can play an important role in the pathogenesis of the rheumatic disease, including rheumatoid arthritis, systemic lupus erythematosus, and polymyositis among others. Besides, enhanced postmyocardial infarction remodeling in type 1 diabetes mellitus was partially mediated by HMGB1 activation.
完全な名称
high mobility group box 1
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参考文献
  • Ulloa L, et al. (2006) High-mobility group box 1 (HMGB1) protein: friend and foe. Cytokine Growth Factor Rev. 17 (3): 189-201.
  • Pisetsky DS, et al. (2008) High-mobility group box protein 1 (HMGB1): an alarmin mediating the pathogenesis of rheumatic disease. Arthritis Res Ther. 10 (3): 209.
  • Volz HC, et al. (2010) The role of HMGB1/RAGE in inflammatory cardiomyopathy. Semin Thromb Hemost. 36(2): 185-94.
  • Sims GP, et al. (2010) HMGB1 and RAGE in inflammation and cancer. Annu Rev Immunol. 28: 367-88.
  • Andersson U, et al. (2010) The role of HMGB1 in the pathogenesis of rheumatic disease. Biochim Biophys Acta. 1799 (1-2): 141-8.
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