Anti-Fibronectin Magnetic Beads Immunoprecipitation (IP) Kit

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抗Fibronectin 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-Fibronectin Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Fibronectin magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗Fibronectin 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-Fibronectin magnetic Beads, conjugated with Anti-Fibronectin antibody, are used for immuneprecipitation (IP) of Fibronectin proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Fibronectin proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Fibronectin proteins are dissociated from the magnetic beads using an elution buffer.

抗Fibronectin 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-Fibronectin Antibody, Rabbit Polyclonal(10314-RP03)
免疫原
Recombinant Human Fibronectin Fragment 2 protein (Catalog#10314-H08H)
交差反応
Human Fibronectin Fragment 2
ソース
Polyclonal Human Rabbit IgG
調製
Produced in rabbits immunized with purified, recombinant Human Fibronectin Fragment 2 (Catalog#10314-H08H; Ser 607-Pro 1265; CAD91166). Fibronectin Fragment 2 specific IgG was purified by human Fibronectin Fragment 2 affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Fibronectin Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-CIGALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-ED-BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FINCALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FNALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FNZALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-GFNDALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-GFND2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-LETSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MSFALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Fibronectin 背景情報

Fibronectin (FN) is a glycoprotein component of the extracellular matrix of the extracellular matrix (ECM) with roles in embryogenesis, development, and wound healing. More recently, FN has emerged as player in platelet thrombus formation and diseases associated with thrombosis including vascular remodeling, atherosclerosis, and cardiac repair following a myocardial infarct. Each monomer of FN consists of three types of homologous repeating units, that is 12 type I repeats, two type II repeats and 15-17 type III repeats. The occurrence of multiple isoforms results from alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. As an ECM component and one of the primary cell adhesion molecules, Fibronectin can be a ligand for fibrin, heparin, chondroitin sulfate, collagen/gelatin, as well as many integrin receptors through which FN mediates the variety of cellular signaling pathways. The study of solid human tumors showed among the early signs of malignant transformation the fragmentation of pericellular FN, concommitent with the increase of its production by the peritumoral stroma. These results should encourage further investigations concerning the potential importance of Fn production and breakdown during cancer progression. FN1 expression has been described to increase significantly from the morula towards the early blastocyst stage, suggesting that FN1 may also be involved in early blastocyst formation. The fragment 2 of FN comprises the first 7 FN type III repeats and is suggested to be important for self association during fibril growth via the key module III2.
完全な名称
fibronectin 1
参考文献
  • Labat-Robert J. (2002) Fibronectin in malignancy. Semin Cancer Biol. 12(3): 187-95.
  • Goossens K, et al. (2009) Quantification of fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos. BMC Dev Biol. 9: 1.
  • Maurer LM, et al. (2010) Emerging roles of fibronectin in thrombosis. Thromb Res. 125(4): 287-91.
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