Anti-Fas Ligand Magnetic Beads Immunoprecipitation (IP) Kit

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抗Fas Ligand 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-Fas Ligand Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-Fas Ligand magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗Fas Ligand 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-Fas Ligand magnetic Beads, conjugated with Anti-Fas Ligand antibody, are used for immuneprecipitation (IP) of Fas Ligand proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing Fas Ligand proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound Fas Ligand proteins are dissociated from the magnetic beads using an elution buffer.

抗Fas Ligand 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-Fas Ligand Antibody, Rabbit Polyclonal(101369-T36)
免疫原
A synthetic peptide corresponding to the center region of the Human Fas Ligand / FASLG / CD95L
交差反応
Human Fas Ligand / FASLG / CD95L
ソース
Polyclonal Human Rabbit IgG
調製
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Fas Ligand / FASLG / CD95L, and purified by antigen affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-Fas Ligand Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-ALPS1BALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-APT1LG1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-APTLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CD178ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CD95-LALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CD95LALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FASLALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-FASLGALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNFSF6ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-TNLG1AALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

Fas Ligand 背景情報

Fas Ligand, also known as FASLG and CD95L, is the ligand for FAS. It is a transmembrane protein which binds to TNFRSF6/FAS. Interaction of FAS with fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Fas Ligand may be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
完全な名称
Fas ligand
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • p53 Pathway
    p53 Pathway
参考文献
  • Pitti R M. et al., 1998, Nature. 396 (6712): 699-703.
  • Hane M. et al., 1995, FEBS Lett. 373 (3): 265-8.
  • Schneider P. et al., 1997, J Biol Chem. 272 (30): 18827-33.
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