Anti-CD19 Magnetic Beads Immunoprecipitation (IP) Kit

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抗CD19 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-CD19 Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-CD19 magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗CD19 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-CD19 magnetic Beads, conjugated with Anti-CD19 antibody, are used for immuneprecipitation (IP) of CD19 proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing CD19 proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound CD19 proteins are dissociated from the magnetic beads using an elution buffer.

抗CD19 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-CD19 Antibody(11880-R303)
免疫原
Recombinant Human CD19 protein (Catalog#11880-H08H)
交差反応
Human CD19
ソース
Monoclonal Human Rabbit IgG
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD19 (rh CD19; Catalog#11880-H08H; P15391; Met1-Lys291).
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-CD19 Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-B4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-CVID3ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

CD19 背景情報

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 19 (CD19) is a member of CD system. CD19 is a cell surface molecule that assembles with the antigen receptor of B-cells. This results in a descent in threshold for antigen receptor-dependent stimulation. A simplified view holds that the ability of B-cells to respond to the various antigens in a specific and sensitive manner is achieved in the presence of low-affinity antigen receptors. CD19 primarily acts as a B-cell coreceptor in conjunction with CD21 and CD81. The formation of the receptor complex is induced by antigen and CD19, induced by exogenous antigen, has been found cytoplasmic tail phosphorylated and bind to sIg.
完全な名称
CD19 molecule
研究分野
Related Pathways
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Carter RH, et al. (1992) CD19: lowering the threshold for antigen receptor stimulation of B lymphocytes. Science. 256 (5053): 105-7.
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