Anti-alpha-Synuclein Magnetic Beads Immunoprecipitation (IP) Kit

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抗alpha-Synuclein 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-alpha-Synuclein Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-alpha-Synuclein magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗alpha-Synuclein 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-alpha-Synuclein magnetic Beads, conjugated with Anti-alpha-Synuclein antibody, are used for immuneprecipitation (IP) of alpha-Synuclein proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing alpha-Synuclein proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound alpha-Synuclein proteins are dissociated from the magnetic beads using an elution buffer.

抗alpha-Synuclein 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Recombinant Anti-alpha-Synuclein Antibody, Rabbit Monoclonal(12093-R017)
免疫原
Recombinant Human SNCA / alpha-Synuclein protein (Catalog#12093-HNAE)
交差反応
Human SNCA / alpha-Synuclein
ソース
Monoclonal Human Rabbit IgG
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human SNCA / alpha-Synuclein (rh SNCA / alpha-Synuclein; Catalog#12093-HNAE; P37840-1; Met1-Ala140).
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-alpha-Synuclein Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-NACPALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PARK1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PARK4ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-PD1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

alpha-Synuclein 背景情報

Alpha-Synuclein (alpha-Syn), also known as NACP or SNCA, exists as at least two structural isoforms: one is helix-rich, membrane-bound form that both the N- and C-terminal regions of alpha-synuclein are tightly associated with membranes and the other is disordered, cytosolic form. Synuclein is found predominantly in the presynaptic termini, in both free or membrane-bound forms. SNCA is extensively localized in nucleus of neurons. It has been shown that alpha-Synuclein was highly expressed in the mitochondria in olfactory bulb, hippocampus, striatum, and thalamus, where the cytosolic alpha-Synuclein was also rich. Normally the unstructured soluble type of alpha-synuclein can aggregate to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson's disease, dementia with Lewy bodies and multiple system atrophy. SNCA abnormality and mitochondrial deficiency are two major changes in the brain of patients with Parkinson's disease (PD). In addition, alpha-synuclein is an abundant component of Lewy bodies in sporadic Parkinson's disease and diffuse Lewy body disease.
完全な名称
synuclein, alpha (non A4 component of amyloid precursor)
参考文献
  • Arima K, et al. (1998) Immunoelectron-microscopic demonstration of NACP / alpha-synuclein-epitopes onthe filamentous component of Lewy bodies in Parkinson's disease and in dementia with Lewy bodies. Brain Res. 808 (1): 93-100.
  • Arima K, et al. (1998) NACP / alpha-synuclein immunoreactivity in fibrillary components of neuronal and oligodendroglial cytoplasmic inclusions in the pontine nuclei in multiple system atrophy. Acta Neuropathol. 96 (5): 439-44.
  • Lee HJ, et al. (2001) Membrane-bound alpha-Synuclein Has a High Aggregation Propensity and the Ability to Seed the Aggregation of the Cytosolic Form. The Journal of Biological Chemistry. 277: 671-8.
  • Alpha-synuclein Dopaminylation Presented in Plasma of Both Healthy Subjects and Parkinson's Disease Patients
    Author
    Zhao, H;Huang, S;Palanisamy, S;Wang, C;Rainer, G;Zhang, X;
    Year
    2020
    Journal
    Proteomics Clin Appl
    Application
    IP
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