Anti-14-3-3 epsilon Magnetic Beads Immunoprecipitation (IP) Kit

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抗14-3-3 epsilon 磁気ビーズ免疫沈降(IP)キットの成分

Components Storage
Anti-14-3-3 epsilon Magnetic Beads1,3 2-8℃ for 12 months
NP40 Cell Lysis Buffer2 -20℃ for 12 months
5×TBST(pH7.4)  
1×TBST(pH7.4)  
ddH2O  
Alkaline Elution Buffer 2-8℃ for 12 months
Acidity Elution Buffer 2-8℃ for 12 months
Neutralization Buffer 2-8℃ for 12 months

【1】The IP KIT contains anti-14-3-3 epsilon magnetic Beads (2 mg/mL) in phosphate buffered saline (PBS, pH 7.4) with sodium azide (0.1%).

【2】Using NP-40 cell lysate buffer in the kit is required,otherwise,the magnetic beads may be precipitated.

【3】Shipping: Magnetic Beads kits are shipped at ambient temperature in which magnetic beads are provided in liquid buffer.

抗14-3-3 epsilon 磁気ビーズ免疫沈降(IP)キットの製品説明

The Anti-14-3-3 epsilon magnetic Beads, conjugated with Anti-14-3-3 epsilon antibody, are used for immuneprecipitation (IP) of 14-3-3 epsilon proteins which expressed in vitro expression systems. For IP, the beads are added to a sample containing 14-3-3 epsilon proteins to form a bead-protein complex. The complex is removed from the solution manually using a magnetic separator. The bound 14-3-3 epsilon proteins are dissociated from the magnetic beads using an elution buffer.

抗14-3-3 epsilon 磁気ビーズ免疫沈降(IP)キット抗体の情報

抗体
Anti-14-3-3 epsilon Antibody(203015-T38)
免疫原
E. coli-derived Human 14-3-3 epsilon/YWHAE fragment
交差反応
Human 14-3-3 epsilon/YWHAE
ソース
Polyclonal Human Rabbit IgG
調製
Produced in rabbits immunized with E. coli-derived Human 14-3-3 epsilon/YWHAE fragment, and purified by antigen affinity chromatography.
アプリケーション
Immunoprecipitation (IP), Minimum Protein Purification

Anti-14-3-3 epsilon Magnetic Beads Immunoprecipitation (IP) Kit: 別名

Anti-14-3-3EALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-HEL2ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-KCIP-1ALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MDCRALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit; Anti-MDSALCAM Magnetic Beads-Immunoprecipitatiopn (IP) Kit

14-3-3 epsilon 背景情報

YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 1% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.
完全な名称
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • TNF Signaling
    TNF Signaling
  • Actin Dynamics Signaling Pathway
    Actin Dynamics Signaling Pathway
  • AKT Signaling Pathway
    AKT Signaling Pathway
  • MAPK-Erk Pathway
    MAPK-Erk Pathway
参考文献
  • Ikeda M, et al. (2008) Identification of YWHAE, a gene encoding 14-3-3epsilon, as a possible susceptibility gene for schizophrenia. Hum Mol Genet. 17(20): 3212-22.
  • Mignon-Ravix C, et al. (2010) Deletion of YWHAE in a patient with periventricular heterotopias and pronounced corpus callosum hypoplasia. J Med Genet. 47(2): 132-6.
  • Nagamani SC, et al. (2009) Microdeletions including YWHAE in the Miller-Dieker syndrome region on chromosome 17p13.3 result in facial dysmorphisms, growth restriction, and cognitive impairment. J Med Genet. 46(12): 825-33.
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