Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein ORF mammalian expression plasmid, C-Flag tag

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Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein ORF mammalian expression plasmid, C-Flag tag: 製品情報

遺伝子
H7N9
遺伝子の長さ
1497 bp
シーケンスの特徴
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with AHZ59787.1.
製品の特徴
Full length Clone DNA of Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein with C terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI (6kb + 1.54kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

Nucleocapsid / NP cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein ORF mammalian expression plasmid, C-Flag tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

Influenza A H7N9 (A/Anhui/1/2013) Nucleoprotein ORF mammalian expression plasmid, C-Flag tag: 別名

NP cDNA ORF Clone, H7N9

Nucleocapsid / NP 背景情報

Influenza A viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation.The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite.

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