TMP21 cDNA ORF Clone, Human, N-DDK (Flag®) tag

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TMP21 cDNA ORF Clone, Human, N-DDK (Flag®) tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
654 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence.
製品の特徴
Full length Clone DNA of Human transmembrane emp24-like trafficking protein 10 (yeast) with N terminal Flag tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI(6kb+0.65kb)
タグシーケンス
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

TMP21 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

TMP21 cDNA ORF Clone, Human, N-DDK (Flag®) tag: 検証済の画像

The plasmid was transfected into 293FT adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

TMP21 cDNA ORF Clone, Human, N-DDK (Flag®) tag: 別名

p23 cDNA ORF Clone, Human; P24(DELTA) cDNA ORF Clone, Human; S31I125 cDNA ORF Clone, Human; S31III125 cDNA ORF Clone, Human; Tmp-21-I cDNA ORF Clone, Human; TMP21 cDNA ORF Clone, Human

TMP21 背景情報

TMED1 disrupts the complex formation between TGF-beta type I (also termed ALK5) and type II receptors (TbetaRII). Misexpression studies revealed that TMED1 attenuated TGF-beta-mediated signaling. A 2-amino acid-long region from Thr(91) to Glu(11) within the extracellular region of TMED1 was found to be crucial for TMED1 interaction with both ALK5 and TbetaRII. Synthetic peptides corresponding to this region inhibit both TGF-beta-induced Smad2 phosphorylation and Smad-dependent transcriptional reporter activity. In a xenograft cancer model, where previously TGF-beta was shown to elicit tumor-promoting effects, gain-of-function and loss-of-function studies for TMED1 revealed a decrease and increase in the tumor size, respectively. That TMED1 expression levels are the key determinant for efficiency of TGF-beta receptor complex formation and signaling.
完全な名称
transmembrane emp24-like trafficking protein 10 (yeast)
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