GRP78 / HSPA5 cDNA ORF Clone, Human, C-OFPSpark® tag

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GRP78 / HSPA5 cDNA ORF Clone, Human, C-OFPSpark® tag: 製品情報

遺伝子
Human
NCBI 参考シーケンス番号
遺伝子の長さ
1965 bp
シーケンスの特徴
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 6 G>A not causing the amino acid variation.
製品の特徴
Full length Clone DNA of Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) with C terminal OFPSpark / RFP tag.
プラスミド
プロモーター
Enhanced CMV promoter
ベクター
制限部位
KpnI + XbaI (6kb + 2.66kb)
タグシーケンス
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
シークエンシングプライマー
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
品質管理
The plasmid is confirmed by full-length sequencing.
スクリーニング
細菌スクリーニング抵抗力
Kanamycin
細胞スクリーニング抵抗力
Hygromycin
アプリケーション
Stable or Transient mammalian expression
保存 & 配送
配送方法
Each tube contains lyophilized plasmid.
保存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

GRP78 / HSPA5 cDNA ORF ヌクレオチド配列およびアミノ酸配列に関する情報

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

GRP78 / HSPA5 cDNA ORF Clone, Human, C-OFPSpark® tag: 検証済の画像

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.
The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.

GRP78 / HSPA5 cDNA ORF Clone, Human, C-OFPSpark® tag: 別名

BIP cDNA ORF Clone, Human; GRP78 cDNA ORF Clone, Human; HEL-S-89n cDNA ORF Clone, Human; MIF2 cDNA ORF Clone, Human
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