Anti-Hsp27 / HSPB1 Antibody

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Anti-Hsp27 / HSPB1 Antibody (Rabbit Monoclonal 抗体) の製品情報

製品名
Anti-Hsp27 / HSPB1 Antibody
検証済のアプリケーション
FCM,ICC/IF
交差反応
Reacts with: Human
特異性
Human Hsp27 / HSPB1
免疫原
Recombinant Human HSP27 protein (Catalog#10351-H08E)
調製
This antibody was obtained from a rabbit immunized with purified, recombinant Human HSP27 (rh HSP27; Catalog#10351-H08E; NP_001532.1; Met1-Lys205).
ソース
Monoclonal Rabbit IgG Clone #155
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Hsp27 / HSPB1 Antibody (Rabbit Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ICC-IF 1:20-1:100
FCM 1:25-1:100
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Hsp27 / HSPB1 Antibody (Rabbit Monoclonal 抗体) の画像

Immunofluorescence staining of Human HSP27 in A431 or SKBR3 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human HSP27 monoclonal antibody (1:60). Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (left panel, captured by laser confocal scanning microscope; right panel, captured by fluorescence microscope), countstained with DAPI (blue). Positive staining was localized to cytoplasm.
Flow cytometric analysis of Human HSP27 on HeLa cells. Cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human HSP27, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.

Anti-Hsp27 / HSPB1 Antibody: 別名

Anti-CMT2F Antibody; Anti-HEL-S-102 Antibody; Anti-HMN2B Antibody; Anti-HS.76067 Antibody; Anti-Hsp25 Antibody; Anti-HSP27 Antibody; Anti-HSP28 Antibody; Anti-SRP27 Antibody

Standard Antibody Development Service

Rabbit MAb

Mouse MAb

Rabbit PAb

Fast Antibody Development Service

Mouse MAb

Rabbit PAb

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