Anti-GFP Antibody

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Anti-GFP Antibody (Mouse Monoclonal 抗体) の製品情報

製品名
Anti-GFP Antibody
検証済のアプリケーション
ELISA,ICC/IF,IP
交差反応
Reacts with: Aequorea victoria
特異性
Aequorea victoria GFP
No cross-reactivity in ELISA with
E.coli cell lysate
免疫原
Recombinant Aequorea victoria GFP protein (Catalog#13105-S07E)
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Aequorea victoria GFP (Catalog#13105-S07E; AAB65663; Ser 2-Lys 238). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #05
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-GFP Antibody (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
ELISA 0.5-1 μg/mL
ICC-IF 10-25 μg/mL
IP 1-4 μg/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-GFP Antibody (Mouse Monoclonal 抗体) の画像

Immunofluorescence staining of GFP protein in CHO cells, transfected with GFP. Cells (left: GFP, middle: antibody, right: merge) were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-GFP monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue).

Anti-GFP Antibody: 別名

Anti-GFP Antibody

GFP 背景情報

The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 58 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
参考文献
  • Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637.
  • Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.
  • Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067.
  • Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028.
  • Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.
  • Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.

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