GAPDH Loading Control Antibody, Mouse Mab

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GAPDH Loading Control Antibody, Mouse Mab (Mouse Monoclonal 抗体) の製品情報

製品名
GAPDH Loading Control Antibody, Mouse Mab
検証済のアプリケーション
WB,IHC-P
交差反応
Reacts with: Human
特異性
Human GAPDH
免疫原
A synthetic peptide corresponding to the center region of the human GAPDH
調製
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the center region of the human GAPDH. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
ソース
Monoclonal Mouse IgG1 Clone #05
精製
Protein A
緩衝液
0.2 μm filtered solution in PBS
標識物
Unconjugated
状態
Liquid
配送方法
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
保存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

GAPDH Loading Control Antibody, Mouse Mab (Mouse Monoclonal 抗体) 検証済のアプリケーション

アプリケーション 推奨希釈率/用量
WB 1:500-1:2000
IHC-P 1:100-1:500
Please Note: Optimal concentrations/dilutions should be determined by the end user.

GAPDH Loading Control Antibody, Mouse Mab (Mouse Monoclonal 抗体) の画像

Anti-GAPDH mouse monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: Hela Whole Cell Lysate

Lane C: MCF7 Whole Cell Lysate

Lane D: MCF7 whole cell lysate

Lane E: PC-3 whole cell lysate

Lane F: Daudi whole cell lysate

Lane G: A549 whole cell lysate

Lane H: Rat heart tissue lysate

Lane I: Rat liver tissue lysate

Lane J: Rat spleen tissue lysate

Lane K: Rat lung tissue lysate

Lane L: Rat kidney tissue lysate

Lane M: Rat brain tissue lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size: kDa

Observed band size:38 kDa

Immunochemical staining of human GAPDH in human renal carcinoma with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human GAPDH in human cirrhosis with mouse monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.

GAPDH Loading Control Antibody, Mouse Mab: 別名

Anti-G3PD Antibody; Anti-GAPD Antibody; Anti-HEL-S-162eP Antibody

GAPDH 背景情報

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
完全な名称
glyceraldehyde-3-phosphate dehydrogenase
参考文献
  • Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
  • Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
  • Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
  • Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
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