TREM-2 ELISA Kit, Human

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TREM-2 ELISA Kit, Human: 製品情報

製品名
TREM-2 ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Recombinant protein
感度
33.48 pg/mL
線形範囲
78.13-5000 pg/mL
特異性
Recognizes both recombinant and natural Human TREM-2
干渉
Preparations of human TREML2 at 50 ng/mL in a mid-range recombinant human TREM2 control were assayed for interference. No significant interference was observed.
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 671.00 1,670.00 2,875.00
SD 64.88 153.00 109.02
CV(%) 9.7% 9.2% 3.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 656.00 1,399.00 2,838.00
SD 63.54 171.09 200.88
CV(%) 9.7% 12.2% 7.1%
回収率
The recovery of Human TREM2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=3)
Average % Recovery
88
Range
81 -99%
直線性
Supernatant
  Recovery of detected
1:1 109%
1:2 91%
1:4 99%
1:8 111%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This TREM-2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human TREM-2 protein in Recombinant protein . It contains recombinant Human TREM-2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

TREM-2 ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Human TREM2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

TREM-2 ELISA Kit, Human: 別名

TREM-2 ELISA Kit, Human; Trem2a ELISA Kit, Human; Trem2b ELISA Kit, Human; Trem2c ELISA Kit, Human

TREM-2 背景情報

Triggering receptor expressed on myeloid cells 2 ( TREM2 ) is a single Ig domain receptor. It is expressed on macrophages and dendritic cells but not on granulocytes or monocytes. Its expression is most abundant in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, and microglial cells are the major TREM2-producing cell type in the central nervous system (CNS). TREM2 may play a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. It also associates with the signal adapter protein, DAP12, which has a cytoplasmic ITAM, leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is both required and sufficient for competent uptake of apoptotic neuronal cells. TREM2 and TREM2-L form a receptor-ligand pair connecting microglia with apoptotic neurons, directing removal of damaged cells to allow repair. Deficiency of the adapter protein DAP12 or its associated receptor TREM2 is associated with abnormal osteoclast development in humans. Defects in TREM2 are causes of PLOSL, also known as NHD. In addition, TREM2 signaling is also an important pathway to promote healing of wounds in the colon where stem cell replacement is necessary.
完全な名称
triggering receptor expressed on myeloid cells 2
参考文献
  • Bouchon, A. et al., 2000, J. Immunol. 164: 4991-4995.
  • Paloneva, J. et al., 2002, Am. J. Hum. Genet. 71:656-662. 
  • Prada, I. et al., 2006, Neuroscience. 140 (4): 1139-48.
  • Neumann, H. et al., 2007, J Neuroimmunol. 184 (1-2): 92-9.
  • Thrash, JC. et al., 2009, Neurochem Res. 34 (1): 38-45.
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