CSF1R ELISA Kit, Human

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CSF1R ELISA Kit, Human: 製品情報

製品名
CSF1R ELISA Kit, Human
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
検出する試料
Serum,Cell supernatant,Plasma
感度
26.93 pg/mL
線形範囲
78.13-5000 pg/mL
特異性
Recognizes both recombinant and natural Human CSF1R
干渉
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human MCSFR control were assayed for interference. No significant interference was observed.
精度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.13 0.31 0.65
SD 0.01 0.01 0.03
CV(%) 5.1% 4.8% 4.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.15 0.30 0.68
SD 0.02 0.02 0.05
CV(%) 10.3% 5.6% 7.5%
回収率
The recovery of Human CSF1R spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
94
Range
92 -99%
直線性
Serum
  Recovery of detected
1:2 90%
1:4 93%
1:8 95%
1:16 89%
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
製品概要
This CSF1R ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CSF1R protein in Serum,Cell supernatant,Plasma . It contains recombinant Human CSF1R, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CSF1R ELISA Kit, Human: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human MCSFR. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CSF1R ELISA Kit, Human: 別名

C-FMS ELISA Kit, Human; CD115 ELISA Kit, Human; CSF-1R ELISA Kit, Human; CSFR ELISA Kit, Human; FIM2 ELISA Kit, Human; FMS ELISA Kit, Human; HDLS ELISA Kit, Human; M-CSF-R ELISA Kit, Human; MCSF Receptor ELISA Kit, Human

CSF1R 背景情報

M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation. However, studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.
完全な名称
colony stimulating factor 1 receptor
参考文献
  • Sherr CJ. (1990) The colony-stimulating factor 1 receptor: pleiotropy of signal-response coupling. Lymphokine Res. 9(4): 543-8.
  • Kacinski BM. (1997) CSF-1 and its receptor in breast carcinomas and neoplasms of the female reproductive tract. Mol Reprod Dev. 46(1): 71-4.
  • Sapi E, et al. (1999) The role of CSF-1 in normal and neoplastic breast physiology. Proc Soc Exp Biol Med. 220(1): 1-8.
  • Sapi E. (2004) The role of CSF-1 in normal physiology of mammary gland and breast cancer: an update. Exp Biol Med (Maywood). 229(1): 1-11.
  • Bonifer C, et al. (2008) The transcriptional regulation of the Colony-Stimulating Factor 1 Receptor (csf1r) gene during hematopoiesis. Front Biosci. 13: 549-60.
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