SARS-CoV-2 (2019-nCoV) Spike Detection ELISA Kit

[FOR RESEARCH USE ONLY.] Online Datasheet is a general version, for batch specific information please refer to the hard copy in shipping package.

SARS-CoV-2 (2019-nCoV) Spike Detection ELISA Kit: 製品情報

製品名
SARS-CoV-2 (2019-nCoV) Spike Detection ELISA Kit
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
感度
77.81 pg/mL
線形範囲
156.3-10000 pg/mL
特異性
Recognizes both recombinant and natural SARS-CoV-2 Coronavirus spike
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Standard Dilution Buffer
7. Color Reagent A
8. Color Reagent B
9. Stop Solution
製品概要
This SARS-CoV-2 (2019-nCoV) Spike Detection ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of SARS-CoV-2 Coronavirus spike protein in . It contains recombinant SARS-CoV-2 Coronavirus spike, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

SARS-CoV-2 (2019-nCoV) Spike Detection ELISA Kit: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Coronavirus spike 背景情報

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.
  • Potent neutralizing antibodies against SARS-CoV-2 identified by high-throughput single-cell sequencing of convalescent patients' B cells
    Author
    Cao, Y;Su, B;Guo, X;Sun, W;Deng, Y;Bao, L;Zhu, Q;Zhang, X;Zheng, Y;Geng, C;Chai, X;He, R;Li, X;Lv, Q;Zhu, H;Deng, W;Xu, Y;Wang, Y;Qiao, L;Tan, Y;Song, L;Wang, G;Du, X;Gao, N;Liu, J;Xiao, J;Su, XD;Du, Z;Feng, Y;Qin, C;Qin, C;Jin, R;Xie, XS;
    Year
    2020
    Journal
    Cell
  • Ultra-sensitive nanozyme-based chemiluminescence paper test for rapid diagnosis of SARS-CoV-2 infection
    Author
    Liu, D;Ju, C;Han, C;Shi, R;Chen, X;Duan, D;Yan, J;Yan, X;
    Year
    2020
    Journal
    bioRxiv
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