SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit

[FOR RESEARCH USE ONLY.] Online Datasheet is a general version, for batch specific information please refer to the hard copy in shipping package.

SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit: 製品情報

製品名
SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit
検出の原理
Solid Phase Sandwich ELISA (quantitative)
標識物
HRP
感度
46.68 pg/mL
線形範囲
93.75-6000 pg/mL
特異性
Recognizes both recombinant and natural SARS-CoV-2 Coronavirus Nucleocapsid
ELISAキット(すぐに使用できる)の成分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Standard Dilution Buffer
7. Sample Lysis Buffer
8. Color Reagent A
9. Color Reagent B
10. Stop Solution
製品概要
This SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of SARS-CoV-2 Coronavirus Nucleocapsid protein in . It contains recombinant SARS-CoV-2 Coronavirus Nucleocapsid, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
配送方法
This ELISA Kit is shipped at ambient temperature.
保存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

SARS-CoV-2 (2019-nCoV) Nucleocapsid Detection ELISA Kit: 画像

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Coronavirus Nucleocapsid 背景情報

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
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