The kit applies for the screening of SARS-CoV-2 (2019-nCoV) inhibitors.
The principle of the kit is competitive ELISA. The SARS-CoV-2 (2019-nCoV) Inhibitor in the samples competes with ACE2-His (Cat: 10108-H08B) to combine with immobilized SARS-CoV-2 S Protein RBD. The signal color becomes lighter as the content of SARS-CoV-2 Inhibitor increases.
|Components||1 Kit (96 Tests)|
|SARS-CoV-2 Inhibitor (Control)||40 μL|
|Human ACE2 (His Tag)||1 vial|
|Anti-His Tag Ab (HRP)||20 μL|
|Microplate: Pre-coated with SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombiant Protein||1 Plate|
|20 × Dilution Buffer||5 mL|
|20 × Wash Buffer||25 mL|
|Color Reagent A||12.5 mL|
|Color Reagent B||12.5 mL|
|Stop Solution||8 mL|
Upon receipt, please store each component at the temperature recommended in datasheet.
This inhibition curve is only for demonstration purposes. A curve should be generated for each assay.
|SARS-CoV-2 Inhibitor (Control) concentration(nM)||mean value OD450nm||percent activity(%)|
The inhibition curve of SARS-CoV-2 Inhibitor (Control) to the binding between RBD and ACE2, IC50=3.90 nM
Detect three monoclonal antibodies by SARS-CoV-2
Inhibitor Screening ELISA Kit (Cat# KIT001)